Accuro Biologics - Custom Antibody Sequencing Service


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	Accurobio™ Custom Antibody Sequencing Service Accuro Biologics has extensive experience in antibody V-region sequence determination from cDNA. We offer a rapid yet high quality and cost effective service. Sequencing is conducted at the level of cDNA and sequences are compiled based on bi-directional sequencing of multiple independent clones. Click to view graphic. The service is applicable to antibodies derived from all common species, including mouse, rat, rabbit and human sources. The service is fully customised but includes the following features: Comprehensive report of protein and DNA V-region sequences CDR annotation (Kabat and Chothia annotation) Antibody gene family sub-group assignment Archive of data files Plasmid DNA storage for future use Projects delivered in 4- 5 weeks Starting Materials Although not critical, we would like to know the isotype of your antibody before we start the project. A pellet of snap frozen cells expressing your antibody should be sent to our laboratory in the UK. Ideally we would prefer to receive around 1x10⁷ cells. Viable cells may be shipped to us to culture if required. Hybridoma Rescue Valuable antibodies may be rescued from non-viable cells, if required. Project Options Accuro Biologics provides an option to achieve complete validation of the V-region sequences by production of a recombinant version of the antibody and testing using your own specific binding assay. Mouse or human constant regions of different isotype may be specified. Cost Effective Service Our comprehensive services are available on flexible and highly competitive terms. Significant discounts are available for multiple antibodies analysed in parallel. Discounts are also available in any follow-on projects commenced with an antibody sequenced by our team. Please contact our sales team to gain a tailored cost for your specific sequencing requirements. Service Summary Accuro Biologics provides an expert antibody engineering group enabling rapid identification of antibody V-regions - rollover to view how you benefit Highly professional and cost effective service Generous discount structure for multiple antibodies in parallel Projects completed in 4 - 6 weeks We provide a high quality and comprehensive report comprising fully annotated antibody protein and DNA sequence data - rollover to view how you benefit No requirement for time consuming bioinformatic analysis or costly software tools Create intellectual property Provide characterising features for further analysis CDRs are identified and fully annotated Encompassed within the service is the provision of an 'engineering feasibility report' itemising the suitability of the antibody for humanization - rollover to view how you benefit Entry point to fast-track humanization programme Rapid and definitive sequence validation study Obligate step towards rapid humanization or other antibody optimization programme Accuro provides an option to create a recombinant version of your antibody featuring your constant region of choice (e.g isotype switching or chimerisation) Accuro applies stringent quality management procedures to every project, your data is fully protected and your V-region DNA is archived for future use